A steam autoclave operates at what pressure and duration to reach 250° F?

The steam autoclave operates effectively at a pressure of 15 pounds per square inch (psi) for a duration of 30 minutes to achieve a temperature of 250°F. This specific combination of pressure and time is crucial for ensuring the sterilization process is both thorough and efficient.

At 15 psi, the steam maintains the necessary temperature for killing a wide range of microorganisms, including bacteria, viruses, and spores, which can be more resistant to lower temperatures. The duration of 30 minutes is also significant; it allows sufficient time for the steam to fully penetrate materials and ensure that the sterilization process is complete, providing an added layer of safety against any surviving pathogens.

Each of the other options reflects variations in pressure and time that do not align with the established effectiveness of steam sterilization practices. Lower pressures or shorter durations can compromise the sterilization efficacy, potentially leading to the survival of harmful microorganisms. Therefore, the combination of 15 psi for 30 minutes is recognized as the standard for effective sterilization in this context.